eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-02-03 4 2 659 664 6074 article Cilem Cimen 1 Halit Demir 1 Aycan Gur 1 Senol Kubilay 1 Science and Arts Faculty, Department of Chemistry, Yuzuncu Yil University, Van - 650 80 (Turkey) Polyphenol oxidase (PPO) was purified from Igdir apricot, with a 367 fold purification of PPO by affinity chromatography being achieved. Amount of the protein was determined according to Bradford method. Vmax and Km values were found by means of Lineweaver- Burk graphs. Asetly salisilic acid, paracetamol, ascorbic acid (vitamin C), sodium sulphate, copper sulphate, glucose, sodium nitrite, sodium chlorure, glisine, sodium azide, 2-merkaptoethanol, tyrosine, citric acid, etilendiamin tetra acetic acid (EDTA) ve p-amino benzoic acid were used as inhibitor. Inhibition constants Ki of each inhibitor were found from Lineweaver-Burk graphs. It was found that the p-aminobenzoik acid function showed the highest inhibitory effect. https://www.biotech-asia.org/vol4no2/investigations-of-the-inhibition-kinetics-of-some-drugs-and-chemicals-on-enzyme-of-polyphenol-oxidase-purified-from-apricots-salak/ Polyphenol oxidase; characterization; purification; inhibitor; kinetics