eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-02-01 4 1 289 292 5751 article Abdullah 1 Shafqat Alauddin 2 Department of Botany, Shibli National P.G. College, Azamgarh - 276 001 (India) Department of Chemistry, Shibli National P.G. College, Azamgarh - 276 001 (India) Wheat and maize were selected to assess the phytotoxic effect of 2, 4-D. The entire experiments were divided into parts. In the first part Seed germination experiment was carried out, where as in the second part Pot culture experiment was done in which plants were grown into polythene bags in the soil and the parameters related to the growth and development were taken on 30th day. The effect of 2, 4-D on seed germination was very evident; as the concentration increased the germination percentage was decreased in both the plants. In the case of wheat, germination was 91% in control followed by 90% in 50 ppm, 87% in 100 ppm and 75% in 150 ppm solutions. For the same solution the germination percentage for the maize were 93, 87, 82 and 72 respectively. The effect of 2, 4-D on time of sprouting of seedling was very pronounced as concentration increased there was delay in the time of sprouting in both the plants. Mean root length and mean shoot length were decreased as concentration increased in the both the plants. The shootlets and rootlets were died on the 15th day in both plants even at 50 ppm solution. In pot culture experiment the leaf area and the number of leaves were decreased as the concentration increases. The photosynthetic pigments i.e. Chlorophyll a and Chlorophyll b were decreased as the concentration increased which indicates the phenomenon of chlorosis. https://www.biotech-asia.org/vol4no1/ex-situ-phyto2-4-d-on-wheat-and-maize/ Phytotoxicity; 2 4-D; Wheat; Maize