eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-02-01 4 1 297 29 5761 article S. Sahoo 1 P.K. Panda 1 S.R. Mishra 1 A. Tripathy 1 P. Ellaiah 2 University Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar -751 004 (India) Pharmaceutical Biotechnology Division, Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam - 530 003 (India) The inhibitory effects of some metal ions, some synthesized compounds and their derivatives with 2-aryl acenaphthimidazoles and their N-Mannich bases on optimum production of hyaluronidase was evaluated employing Streptococcus mitis MTCC *2695 and Streptococcus equi SED 9 by submerged fermentation. The maximum enzymatic activity was obtained with an initial pH (6 and 5.5), incubation temperature 37°, incubation time for 48 h and inoculum level (6 and 10 %) with inoculum age 24 h by Streptococcus mitis and Streptococcus equi SED-9 respectively. The results indicated that metals like Fe3+, Zn2+ Mn2+, Ag+, Ni2+, Sr2+ and Cu2+ acted as strong inhibitors of the enzyme. N-Mannich bases of 2-aryl acenaphthimidazoles exhibited promising inhibitory activity. https://www.biotech-asia.org/vol4no1/effect-of-some-inhibitors-on-hyaluronidase-production-by-streptococcus-mitis-mtcc-2695-and-streptococcus-equi-sed-9-species/ Hyaluronidase production; Streptococcus sp.