eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-02-01 4 1 285 288 5748 article Nishant Mishra 1 Anuraag Mohan 1 U. S. Mishra Phytochemistry Lab, Department of Chemistry, Bareilly College, Bareilly - 243 001 (India) A study was conducted with seeds of barley, Hordeum vulgare to elucidate the effect of bio-fertilizers, applied individually and in conjunction on bio-chemical parameters such as total nitrogen, total protein, total soluble carbohydrates and total lipid contents and macronutrients like calcium, iron and phosphorus under pot culture conditions. A Positive and significant results were obtained by different biofertilizer treatments as compared to their respective controls. Application of Azotobacter showed significant increase in total nitrogen, crude protein, carbohydrate and lipid contents of seeds, whereas Azospirillum significantly increased calcium and iron contents of barley seeds. Combined treatment (Azotobacter+ Azospirillum+ Phosphate Solubilizing Bacteria) also increased seed’s total nitrogen, crude protein, iron, calcium and phosphorus contents as compared to the control. https://www.biotech-asia.org/vol4no1/effect-of-bio-fertilizers-on-bio-chemical-and-macronutrients-of-barley-seeds/ Azospirillum; Azotobacter; Phosphate Solubilizing Bacteria; PSB; Barley; Bio-fertilizers; Macr. Ho