eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-01-28 3 2 341 345 5145 article A. G. Namdeo 1 D. P. Fulzele 2 S. Patil 3 S.S. Kadam 4 Department of Pharmacognosy & Phyto-Biotechnology, Poona College of Pharmacy, BVDU, Erandwane Educational Campus, Pune (India) Biotechnology Division, Bhabha Atomic Research Centre, Mumbai (India) School of Life Sciences, DAVV, Indore (India) Sucrose is a major ingredient of culture medium. Present study was performed to investigate the influence of sucrose concentration on growth and ajmalicine production. Maximum packed cell volume (72%), fresh weight (65g 100 ml-1), and dry weight (6.2148g 100 ml-1) was recorded in medium containing 4% sucrose. About 9-fold increase in dry weight was recorded in medium with 4% sucrose. All growth parameters increased upto 25th day of incubation after which a gradual decline was observed. Ajmalicine accumulation started from 15th day of incubation and reached to maximum (60 μg g-1 dry weight) in 25th day of incubation in medium with 4% sucrose, which however gradually reduced on further incubation. https://www.biotech-asia.org/vol3no2/influence-of-sucrose-concentration-on-growth-and-ajmalicine-production-from-catharanthus-roseus-suspension-cultures-2/ Catharanthus roseus; sucrose; ajmalicine; suspension culture