eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-01-11 3 1 89 93 3574 article T. Sathish 1 U. Kiran Babu 1 K. Kishore Kumar 1 N. Pratap 2 M. Krishna Prasad 1 Center for Biotechnology, Department of Chemical Engineering, Andhra University, Visakhapatnam - 530 003 (India) Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam - 530 003 (India) Silymarin, as the plant active ingredient of milk thirsle a polyphenolic antioxidant flavanoids, protecting the liver cells membrane and facilitate the recovery of the impaired liver cells. Very few analytical methods have been reported for the determination of Silymarin Estimation of Silymarin with (MBTH & CAS) in pure & pharmaceutical dosage form (Method-I), with fecl3 and 2-2 Bipyrdine (Method – II) by using Elico – uv – Visible- spectrophotometer model SL-150. The concentration range 5-40 mg/ml was obeying beer-lambert’s law, at 520 nm in Method – I and concentration range 5- 25 mg/ml was obeying beer-lambert’s law at 525 nm in Method – II. In case of pharmaceutical dosage forms, the silymarin percent content was found to be 99.50 in both the methods. The proposed methods are applicable for the assay of silymarin in both pure and pharmaceutical dosage forms. https://www.biotech-asia.org/vol3no1/new-spectrophotometric-methods-for-the-estimation-of-silymarin-in-pure-and-pharmaceutical-dosage-forms/ Silymarin; MBTH (3-Methyl Benzthia-zolinone-2-Hydrozone)