eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2016-01-11 3 1 85 88 3572 article A. B. Dhake 1 M. B. Patil 1 University Department of Biochemistry, L.I.T. Premises, Nagpur University, Nagpur - 440 033 (India) Penicillium purpurogenum was found to produce cell wall bound b-glucosidase reaching to its peak after 4 days of incubation, which parallels with the growth of fungus. The cell wall bound enzyme exhibited optimum activity at pH 5.5 & 50°C. The ionic detergent SDS at 0.1% concentration shows maximum solubilization of cell wall bound enzyme in to the extraction buffer. The enzyme activity was mildly enhanced by metal ions like K+, Na2+ and strongly inhibited by Hg2+. EDTA shows little inhibition of the b-glucosidase activity. https://www.biotech-asia.org/vol3no1/investigation-in-to-the-cell-wall-bound-beta-glucosidase-of-penicillium-purpurogenum/ b-glucosidase; SDS; Penicillium purpurogenum