eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-08-19 23 3 59581 article Sérgio Dantas de Oliveira Júnior 1 Larissa Batista do Nascimento Soares 2 Aldenora dos Santos Vasconcelos 3 Lorena Viera Bentolila de Aguiar 4 Luciano Henrique Campestrini 5 Jaqueline Araújo Bezerra 6 Molecular Biology Laboratory, Embrapa Western Amazon, Amazonas, Brazil Edible Fungus Cultivation Laboratory, National Institute for Amazonian Research (INPA), Amazonas, Brazil Postgraduate Program in Biodiversity and Biotechnology of the BIONORTE network (PPGBIONORTE), School of Health Sciences (ESA), University of Amazonas (UEA), Amazonas, Brazil Multi-User Center for Analysis of Biomedical Phenomena (CMABio), State University of Amazonas (UEA), Amazonas, Brazil Cellular Zootechnics Laboratory, Agricultural Sciences Sector, Paraná, Brazil Coordination of Technology and Innovation (COTEI), National Institute for Amazonian Research (INPA), Amazonas, Brazil The genus Ganoderma is widely recognized for its therapeutic properties, mainly attributed to bioactive polysaccharides. This study investigated the production and characterization of exopolysaccharides (EPS) from Ganoderma sichuanense (CC22) cultivated by submerged fermentation, evaluating the effects of different carbon sources (glucose and sucrose) at concentrations of 20, 30, and 40 g/L. Glucose at 30 g/L resulted in the highest mycelial growth (8.27 ± 0.01 g/L), while sucrose at 20 g/L showed the lowest performance (2.32 ± 0.01 g/L). The highest EPS yield (4.53 ± 0.01 g/L) was obtained by the freeze-thaw method in a medium with glucose at 40 g/L. Structural analyses of the exopolysaccharide (SEM, FTIR, XRD, UV-Vis, and NMR) confirmed crystalline-amorphous characteristics and the presence of β-glycosidic linkages. The results demonstrate that the choice of carbon source and precipitation method directly influences the quantity and structure of EPS, reinforcing its potential for pharmaceutical and food applications. https://www.biotech-asia.org/vol23no3/development-the-exopolysaccharides-production-from-ganoderma-sichuanense-by-submerged-fermentation/ Basidiomycetes; Bioactive Compounds; Biopolymers; Polysaccharide Production; White-rot Fungi