eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-06-18 23 2 59100 article Pallavi Ashok Vallamdeshi 1 Kishor Vasant Otari 1 Ajay Yashvant Kale 1 Department of Pharmacology,Navsahyadri Institute of Pharmacy, Pune, India The chick embryo model is a widely used in vivo experimental system in developmental biology and biomedical research. This review aims to systematically evaluate the applicability of the chick embryo model in studying vertebrate development, disease mechanisms, and therapeutic interventions. The model is characterized by ex-ovo development, rapid organogenesis, cost-effectiveness, and ethical acceptability, making it a valuable alternative to mammalian systems. This review summarizes key aspects including embryonic staging, major developmental processes, and the role of extra-embryonic membranes, particularly the yolk sac membrane and chorioallantoic membrane, in applications such as angiogenesis, tumor biology, pharmacokinetics, and toxicological assessment. Additionally, commonly used experimental approaches, drug delivery routes, and advanced imaging techniques enabling real-time and longitudinal analysis are discussed. The chick embryo model offers significant advantages in terms of accessibility and experimental flexibility; however, physiological and anatomical differences from mammals limit direct translation to human systems. Therefore, findings obtained from this model should be interpreted cautiously and validated using higher animal models. In conclusion, the chick embryo remains a reliable and ethically acceptable platform for preliminary in vivo investigations, with ongoing technological advancements expected to further enhance its relevance in translational biomedical research. https://www.biotech-asia.org/vol23no2/the-chick-embryo-model-from-developmental-biology-to-comprehensive-biomedical-research-insights/ Angiogenesis; Biomedical research; Chick embryo; Chorioallantoic membrane (CAM); Developmental biology; Embryogenesis