eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-06-25 23 2 59311 article Kouame N´zebo Desire 1 Moumouny Traore 2 Benie Comoe Koffi Donatien 1 Atobla Koua 1 Dadie Adjehi 3 Laboratory of Biotechnology Agriculture and Valorization of Biological Resource, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Côte d’Ivoire Laboratory of Food Microbiology and Biotechnology, Department of Biochemistry and Genetics, Peleforo Gon Coulibaly University, Korhogo, Côte d’Ivoire Laboratory of Food Microbiology and Biotechnology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire This investigation sought to ascertain the ability of Pseudomonas aeruginosa and Escherichia coli isolated from contaminated smoked fish to form biofilms and engage in quorum sensing. Sixty strains of Pseudomonas aeruginosa and forty strains of Escherichia coli isolated from smoked fish were used to conducted this study. The strains were first revived and then inoculated onto Cetrimide and Tryptone bile X glucuronide media. Quorum sensing (LasI and LasR) and biofilm formation gene such as ppyR, PelA and PslA were detected by PCR methods. In P. aeruginosa strains, genes Las were detected with a prevalence of 75% for LasI and LasR. The prevalence of Rhl genes was 58.33% for RhlI and 50% for RhlR, respectively. In E. coli strains, the prevalence was 71.42% for the genes (RhlI/R) and 42.85% for the genes (LasI/R). The biofilm-forming genes PslA, ppyR and PelA were detected in Pseudomonas aeruginosa strains with respective percentages of 50%, 41.66%, and 58.33%. Only PelA (14.28%) and PslA (28.57%) were detected in Escherichia coli strains. Pseudomonas aeruginosa and Escherichia coli are involved in biofilm formation and quorum sensing. Smoking techniques and smoked fish preservation methods need to be improved. https://www.biotech-asia.org/vol23no2/prevalence-of-biofilm-formation-and-quorum-sensing-genes-in-pseudomonas-aeruginosa-and-escherichia-coli-isolated-from-smoked-fish/ Biofilm; Escherichia coli; Pseudomonas aeruginosa; Quorum sensing; Smoked fish