eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-06-17 23 2 59073 article Renji Varghese 1 Jinu John 2 Rogimon Plammoottil Thomas 1 Department of Botany, CMS College Kottayam (Autonomous), Kerala, India. Department of Biotechnology, CMS College Kottayam (Autonomous), Kerala, India. Plant tissue culture serves as a vital biotechnological approach for conserving and micropropagating medicinally significant plant species in India. This study investigates the in-vitro regeneration potential of Cyclea peltata (Lam.) Hook.f. & Thoms.and Tiliacoraacuminata (Lam.) Hook. f. & Thoms. using standard Murashige and Skoog (MS) media. Leaf explants from C. peltata on MS medium fortified with 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (1:1 ratio) produced callus within 14 days of culture. Subsequent transfer of this callus to MS with indole-3-acetic acid (IAA) and kinetin (0.5:5 ratio) induced indirect organogenesis, featuring meristemoid formation and shoot primordia. Additional subculturing on MS supplemented with benzylaminopurine (BAP) and IAA (10:1 ratio) promotedshoot multiplication and average 6 cm elongation in 50% of cultures. Direct shoot regeneration from stem explants with apical meristems on MS + BAP and kinetin (10:1) was delayed, occurring after 5 months. For T. acuminata, nodal explants on MS + BAP and IAA (10:1) exhibited initial morphogenic responses and meristematic activity asnodal swelling of 0.7 cm by 28 days, though mature shoots had not yet formed. These findings demonstrate efficient callus-based organogenesis in C.peltata through phased 2,4-D-kinetin, IAA-kinetin and BAP-IAA regimens, alongside preliminary responsiveness in T. acuminata that warrants refinement of hormone balances and culture parameters. The outlined protocols lay the groundwork for scalable micropropagation and germplasm preservation of these valuable medicinal climbers in conventional Indian tissue culture facilities. https://www.biotech-asia.org/vol23no2/micropropagation-and-tissue-culture-approaches-in-cyclea-peltata-lam-hook-f-and-thoms-and-tiliacora-acuminata-lam-hook-f-and-thoms/ Cyclea peltata; Micropropagation; MS medium; Tiliacora acuminata;Tissue culture