eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-03-30 23 1 294 304 10.13005/bbra/3498 58304 article Nisha Nisha Bhavan Chandran 1 Rogimon Plammoottil Thomas 2 Department of Botany, Assumption College (Autonomous), Changanassery, Kerala, India. Department of Botany, CMS College Kottayam (Autonomous), Kerala, India. India's rich biodiversity is complemented by its vast knowledge of medicinal plants and traditional practices, with plant-based compounds having been harnessed as therapeutic agents for centuries. Cancer, a leading cause of global mortality, is fundamentally driven by DNA abnormalities that disrupt the function of key metabolic proteins. Ras proteins show a vital role in cell signalling, regulating cell proliferation and apoptosis, but mutations in these proteins may results in uncontrolled cell growth. This study investigated the anticancer potential of phytochemicals against mutated H-RAS, N-RAS, and K-RAS (RAS proteins) using molecular docking analysis. Aegle marmelos, rich in over a hundred phytochemicals with reported anticancer properties, was the source of 89 molecules screened as ligands. Seventy-eight molecules exhibited strong binding affinity (∆G_bind ≤ -5 kcal/mol) for H-Ras, 74 for K-Ras, and 77 for N-Ras, with α-amyrin and lupeol emerging as top leads for H-Ras, betulinic acid, β-amyrin, and lupeol for K-Ras, and βand α-amyrins for N-Ras. α-Amyrin emerged as a promising lead molecule against mutated Ras proteins, showing strong potential against H-Ras and N-Ras, and comparable efficacy to top leads against K-Ras. To overcome the challenges observed in drug likeness prediction connected with alpha amyrin, nano emulsion formulations strategies can improve its bioavailability and efficacy. Further validation of the lead molecules' anticancer efficacy requires comprehensive preclinical and clinical studies to confirm their biological activity. https://www.biotech-asia.org/vol23no1/virtual-screening-for-lead-molecules-in-aegle-marmelos-l-correa-on-mutated-ras-proteins/ Aegle marmelos; Anticancer; Auto Dock; Molecular docking; Phytochemicals; Ras proteins