eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-03-30 23 1 402 410 10.13005/bbra/3507 58533 article Parvathy Menon PR 1 Nirmala Devi N 1 Department of Biochemistry, Sree Narayana Guru College, KG Chavadi, Tamil Nadu, India. Kalanchoe pinnata (Lam.) Pers.: a medicinal plant traditionally used for various ailments, has gained attention for its curing various infectious diseases. This plant being a succulent herb with various medicinal applications for several diseases especially cancer have shown its mechanism by several approaches. This herb contains gallic acid, caffeic acid, coumaric acid, stigmasterol, campesterol, and other elements; it is these phytochemicals that participate in the regulation of cell proliferation, regulation, oxidative stress, and apoptosis. They also have the potentiality to act as a drug agents for tumours resistance therapy. Cancer is a global health problem that is associated with the life style changes. Out of which pancreatic cancer represents the most lethal among all; despite being all the advances made in these years. This present work assessed the effect of Kalanchoe pinnata (Lam.) Pers.: extract against the human pancreatic cancer cell line PANC-1 using flow cytometry based analyses. PANC-1 cells were treated with the increasing concentrations of Kalanchoe pinnata (Lam.) Pers. :( 150-450µg/ml). Cell viability were examined using the MTT assay while the cell cycle and apoptosis were determined using the flow cytometry. The results indicated the significance in the increase of cytotoxicity; decrease in the cell viability with the change of concentration. Flow cytometric analysis were carried out for the induction of the apoptosis which demonstrated that the extracts were capable for the induction of cell cycle arrest. The Study outcomes suggests that Kalanchoe pinnata (Lam.) Pers.: exhibits potent in vitro anticancer activity against PANC-1 cells through dose-dependent induction of apoptosis. https://www.biotech-asia.org/vol23no1/in-vitro-anticancer-potential-of-kalanchoe-pinnata-lam-pers-against-panc-1-cells-assessed-by-flow-cytometry/ Apoptosis Cytotoxicity; Flow cytometry; Kalanchoe pinnata (Lam.) Pers.; Pancreatic cancer; PANC-1 cells