eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-03-30 23 1 438 451 10.13005/bbra/3510 58703 article Kalyani Deepak Salunke 1 Rupali Seth 1 Department of Botany, Fergusson College (Autonomous), Pune, India The present study was conducted to evaluate the impact of different seed pretreatment agents on germination and seedling growth in Lablab purpureus (L.) Sweet (variety Kokan Bhushan), under in vitro saline conditions. Five different pretreatments were provided to the seeds: distilled water, NaCl (100 mM), NaCl (500 mM), KCl (100 mM) and KCl (500 mM). Untreated seeds served as control.  The pretreated and untreated seeds were germinated in vitro on half strength Murashige and Skoog’s basal medium supplemented with graded series of salt stress (0, 60, 80 and 100 mM). The germinated seedlings were maintained in saline stress for six weeks. The results demonstrated that pretreatments with distilled water, NaCl (100 mM) and KCl (100 mM) significantly enhanced the germination percentage, shoot and root length, fresh and dry weight of the seedlings both in presence and absence of salinity stress. Pretreated seeds also exhibited improved seedling vigour index and salt tolerance indices. Seed pretreatment with KCl (100 mM) proved most effective followed by NaCl (100 mM) and distilled water. This study substantiates that KCl and NaCl seed pretreatments optimized under in vitro conditions, represent a simple, cost-effective and practical approach in Lablab purpureus (L.), for upscaling to salinity affected fields. https://www.biotech-asia.org/vol23no1/impact-of-seed-pretreatments-in-lablab-purpureus-l-sweet-seedlings-for-mitigating-in-vitro-salinity-stress/ In vitro; KCl; Lablab purpureus (L.); NaCl; Salt stress; Seed pretreatment