eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-03-30 23 1 129 140 10.13005/bbra/3485 58511 article Sarumathi Chandrasekaran 1 Kannahi Manoharan 1 Department of Microbiology, Sengamala Thayaar Educational Trust Women’s College (Autonomous), Affiliated to Bharathidasan University, Sundarakkottai, Mannargudi, Tamilnadu, India. In recent years, growing scientific attention has focused on the therapeutic potential of probiotics, particularly strains belonging to the genera Lactobacillus and Bifidobacterium, in promoting human health. They contribute to the modulation of gut microbiota and are widely used in functional foods. Emerging evidence indicates that probiotics play an important role in the prevention and management of gastrointestinal disorders, including inflammatory bowel disease, irritable bowel syndrome and ulcerative colitis. In addition, probiotics show promise in preventing obesity and in managing type 2 diabetes and cardiovascular diseases through the regulation of host metabolism and inflammatory responses. Probiotics are also implicated in the eradication of Helicobacter pylori and in reducing the risk of gastric ulcer development. This review summarizes findings from peer-reviewed articles published between 2014 and 2025, identified through major scientific databases, that investigate probiotics and their therapeutic applications. Overall, probiotics appear to be promising adjunctive therapeutic agents for gastrointestinal, metabolic and cardiovascular conditions. However, probiotic effects are highly strain-specific and are influenced by dosage, host factors and disease status. Although existing data are promising, further well-designed, large-scale clinical trials are required to establish their efficacy, safety and standardized therapeutic guidelines. https://www.biotech-asia.org/vol23no1/exploring-the-health-benefits-of-probiotics-current-insights-and-therapeutic-applications/ Bifidobacterium; Gut microbiota; Health benefits; Lactobacillus; Probiotics