eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2026-03-30 23 1 41 50 10.13005/bbra/3479 58348 article Suresh Basavaraj Arakera 1 Vinuta Hanamant Mane 1 Shubhangi Pingle 2 Molecular Medicine and Microbial Genetics Laboratory, Department of Applied Genetics, Karnatak University, Pavatenagar, Karnataka, India. Regional Occupational Health Centre, ICMR Institute Devanahalli Bengaluru, Karnataka, India. Lectins are carbohydrate-binding proteins widely distributed in leguminous plants and are known for their hemagglutination and diverse biological activities; in this study, a mannose-specific lectin was isolated and partially characterized from Trigonella foenum-graecum seeds by extraction with phosphate-buffered saline, partial purification through ammonium sulphate precipitation and dialysis, and characterization using hemagglutination, sugar inhibition assays, pH and temperature stability tests, and SDS-PAGE analysis. The lectin exhibited strong hemagglutination activity against human A, B, and O erythrocytes without strict blood-group specificity, and its activity was selectively inhibited by mannose, confirming its mannose specificity. The lectin remained stable within a pH range of 6–8 and at temperatures up to 70°C, while SDS-PAGE revealed a protein band of approximately 28–30 kDa. Overall, the findings indicate that Trigonella foenum-graecum seeds are a rich source of a stable mannose-specific lectin with significant potential applications in glycobiology and biomedical research. https://www.biotech-asia.org/vol23no1/characterization-of-a-mannose-binding-seed-lectin-from-trigonella-foenum-graecum-with-broad-erythrocyte-agglutination/ Hemagglutination; pH; Sugar specificity; Temperature stability; Trigonella foenum-graecum