eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2025-12-30 22 4 1462 1474 10.13005/bbra/3454 57190 article Sameena Begum 1 Niranjan Panda 2 Chinthala Praveena 3 Department of Pharmacy, Chaitanya Deemed to be University, Warangal, India School of Pharmacy, The Neotia University, 24 Parganas (South), West Bengal. India Department of Pharmacy, Chaitanya Deemed to be University, Warangal, India Nanoparticles made from protein-based materials are showing great potential as drug delivery systems because of their special characteristics, such as biodegradability, biocompatibility, and the capacity to hold different therapeutic agents. Among these substances, bovine serum albumin (BSA), a globular protein with superior drug-binding properties, stands out. The manufacturing methods and medicinal uses of BSA nanoparticles are explored in this comprehensive review. The preparation techniques covered in this discussion include self-assembly, desolvation, emulsification, thermal gelation, Nanoparticle albumin-bound (NAB) technology, and nanospray drying. Each of these techniques has unique benefits in terms of drug loading capacity, controlled release characteristics, and particle stability. The applications of BSA nanoparticles in medication delivery systems, cancer therapy, and medical imaging demonstrate their versatility. In drug delivery, they provide controlled release and targeted delivery, enhancing therapeutic efficacy while minimizing side effects. Their capacity to improve chemotherapeutic drug distribution and expedite gene therapy highlights their significance in cancer therapy. Furthermore, the diagnostic capacities are improved by the use of BSA nanoparticles in imaging modalities, including fluorescence and Magnetic Resonance Imaging (MRI). BSA nanoparticles have many benefits, but they also have drawbacks, such as immunogenicity, a low drug loading capacity, stability problems, and size fluctuation. For its wider clinical use, these drawbacks must be addressed. This review highlights the potential of BSA nanoparticles in revolutionising drug delivery and therapeutic strategies, underscoring the need for further research to address existing challenges and optimise their clinical utility. https://www.biotech-asia.org/vol22no4/a-systematic-review-on-bovine-serum-albumin-nanoparticle-preparation-and-therapeutic-application/ Biodegradability; Bovine Serum Albumin; Desolvation; Emulsification; Nanoparticles