eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2025-09-30 22 3 1054 1070 10.13005/bbra/3423 56227 article Anju Sivasankaran 1 Sarada Jonnalagadda 1 Department of Microbiology, Bhavan’s Vivekananda College of Science, Humanities and Commerce, Sainikpuri, India. The biofilm formation in P. aeruginosa is controlled by a network of signalling systems well-known as quorum sensing. This QS system is known to control the virulence factors like production of siderophores, rhamnolipids, exopolysaccharide matrix and motility. Pseudomonas infections have been proven to be resistant to most of the conventional antibiotics and their susceptibility is achieved only at their high concentrations. Since the development of biofilm in P. aeruginosa is a consequence of multiple pressures that has triggered resistance to antibiotics, targeting biofilm needs to be based on mode of action that can weaken more than one of its features. Biogenic Silver Nanoparticles (BSNPs) have been proved to have the highest antimicrobial property and highest level of biocompatibility when compared to other metal nanoparticles. In the present study Biogenic Nano Formulation (BgNF) was developed as an antibiofilm agent which showed 96% of   biofilm inhibition and 89.93% of biofilm dispersal.  BgNF could inhibit exopolysaccharide matrix, and rhamnolipids production by 100%. Siderophores reduction was noted to be 95% for PVD while 92% was observed for PCH. Swarming motility also was found to be inhibited by 69%.  Absence of QS signals was also identified by UPLC-MS analysis. https://www.biotech-asia.org/vol22no3/nano-based-antibiofilm-strategy-against-pseudomonas-aeruginosa/ Biofilm; Biogenic Nanoformulation; Biogenic Silver nanoparticles; Chromobacterium violaceum4212; Pseudomonas aeruginosa YU-V10