eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2025-09-30 22 3 1243 1251 10.13005/bbra/3437 56336 article Barkathulla Syed Nazeer 1 Vijaya Surya Velu 1 Dheepan George 1 Deptartment of Microbiology, AVS College of arts and science (Autonomous), Salem India. To explore the potential of Plant Growth-Promoting Bacteria (PGPB) such as Acidithiobacillus sp. in improving sugarcane tolerance to abiotic stresses like drought, salinity, and nutrient deficiencies, and optimizing carbon supply for sustainable cultivation.¹ The use of Plant Growth-Promoting Bacteria (PGPB) has the potential for enhancing agricultural sustainability and productivity. PGPB supports plants in a variety of ways, including nutrient absorption, hormone production, and disease prevention. However, strain variability, formulation and distribution concerns, regulatory barriers, and socioeconomic constraints all offer obstacles to their widespread use. Addressing these challenges requires collaborative efforts from researchers, policymakers, extension workers, and farmers to identify effective PGPB strains, develop stable formulations, navigate regulatory processes, and provide technical support for successful integration into agricultural practices.² Sugarcane plantlets in tissue culture survived heat stress, suggesting the bioformulation induces abiotic stress tolerance and carbon content was higher in tissue culture-treated, bio-inoculated plants compared to pot culture plants and controls. Despite these challenges, using PGPB has the potential to boost crop yields, minimise chemical inputs, and promote sustainable agriculture, therefore contributing to global food security and environmental stewardship.³ The study To explore the potential of Plant Growth-Promoting Bacteria (PGPB) such as Acidithiobacillus sp. in improving sugarcane tolerance to abiotic stresses like drought, salinity, and nutrient deficiencies, and optimizing carbon supply for sustainable cultivation.¹ The use of Plant Growth-Promoting Bacteria (PGPB) has the potential for enhancing agricultural sustainability and productivity. PGPB supports plants in a variety of ways, including nutrient absorption, hormone production, and disease prevention. However, strain variability, formulation and distribution concerns, regulatory barriers, and socioeconomic constraints all offer obstacles to their widespread use. Addressing these challenges requires collaborative efforts from researchers, policymakers, extension workers, and farmers to identify effective PGPB strains, develop stable formulations, navigate regulatory processes, and provide technical support for successful integration into agricultural practices.² Sugarcane plantlets in tissue culture survived heat stress, suggesting the bioformulation induces abiotic stress tolerance and carbon content was higher in tissue culture-treated, bio-inoculated plants compared to pot culture plants and controls. Despite these challenges, using PGPB has the potential to boost crop yields, minimise chemical inputs, and promote sustainable agriculture, therefore contributing to global food security and environmental stewardship.³ The study suggests that bioformulation induces abiotic stress tolerance and improves carbon supply in sugarcane. https://www.biotech-asia.org/vol22no3/investigating-the-enhancement-of-tolerance-to-abiotic-stress-and-improvement-of-carbon-supply-in-sugarcane-using-acidithiobacillus-sp/ Food security; Hormone synthesis; Plant Growth-Promoting Bacteria; Sustainable agriculture