eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2025-06-25 22 2 675 685 10.13005/bbra/3393 54842 article Kishori Laxman Deore Gokul Shravan Talele Department of Pharmaceutical Chemistry, Matoshri College of Pharmacy, Eklahare, Near Odhagaon Nashik, (MS), India The aim of this research was to create a novel, quick, and precise UV-spectroscopic method for the quantification of Ibrutinib in both its pure form and in capsule dosage form. A straightforward ultraviolet spectrophotometric method was designed and validated based on several parameters, including linearity, precision, reproducibility, accuracy, robustness, ruggedness, as well as the LOD and LOQ. Methanol served as the diluent. The maximum absorbance was observed at 260 nm, and the linearity was discovered to be between 8.00-12.00 μg/ml. The regression equation for Ibrutinib was y = 0.0533x + 0.0356, with a correlation coefficient (R²) of 0.9998. The recovery percentage ranged between 98% and 102%. The RSD for both intraday and interday precision was under 2%. The LOD and LOQ were calculated to be 0.08 µg/mL and 0.24 µg/mL, respectively. The method validation was judged appropriate for regular quantitative analysis of Ibrutinib in both its pure and capsule dose forms in compliance with recommendations established by the International Conference on Harmonization (ICH). https://www.biotech-asia.org/vol22no2/method-development-and-validation-of-a-uv-spectroscopic-approach-for-the-investigation-of-ibrutinib-in-bulk-and-dosage-forms-for-pharmaceuticals/ Ibrutinib; Method Development; Methanol; UV-Spectroscopy; Validation