eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2025-06-25 22 2 649 663 10.13005/bbra/3391 55419 article Jaya Laxman Dolnar 1 Gautam Vithobaji Zodape 1 Department of Zoology, L.S. and S.S. Patkar College of Arts and Science and V. P. Varde College of Commerce and Economics, S.V. Road, Goregaon (West), Mumbai, Maharashtra, India. The research explores the biomedical properties of the box jellyfish Chiropsoides buitendijki, collected from Edwan village, Mumbai. The crude extract of the box jellyfish Chiropsoides buitendijki exhibits a range of bioactive properties with potential biomedical applications. Hemolytic activity observed in both human and chicken red blood cells indicates the presence of toxic proteins. The chick Chorio-Allantoic Membrane (CAM) assay revealed anti-angiogenic effects, including blood vessel damage and inhibition of neovascularization, suggesting potential use in cancer therapy. Neuromodulatory effects were assessed in Sprague-Dawley rat brains, where increased extract concentrations elevated Na⁺/K⁺-ATPase activity and cholinesterase inhibition, implicating potential impacts on metabolic stability and cognitive function. Protein analysis revealed a moderate protein content (0.121 mg/mL). Partial purification was achieved through SDS-PAGE electrophoresis, and the proteins were identified at various 11 kDa to 245 kDa levels, Functional characterization linked specific bands to hemolytic, antimicrobial, immunological, and anti-inflammatory activities. These findings underscore the pharmacological potential of Chiropsoides buitendijki extract, supporting its further exploration as a source of novel compounds for pharmaceutical and biomedical applications. https://www.biotech-asia.org/vol22no2/biomedical-applications-of-chiropsoides-buitendijki-crude-extract-exploring-hemolytic-anti-angiogenic-and-neuromodulatory-properties-for-therapeutic-potential/ Anti-angiogenic; Cytotoxic; Hemolytic; Neuromodulatory; Proteins