eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2025-03-25 22 1 401 410 10.13005/bbra/3371 54676 article Ajay Kumar Shukla 1 Aarti Tiwari 2 Vimal Kumar Yadav 1 Vishnu Prasad Yadav 1 Kunal Agam Kanaujia 1 Manoj Kumar Mishra 2 Institute of Pharmacy, Dr. Ram Manohar Lohia Avadh University, Ayodhya, Uttar Pradesh, India Department of Pharmacy, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, India Shambhunath Institute of Pharmacy, Jhalwa, Prayagraj-211015, Uttar Pradesh, India Pacific College of Pharmacy, Pacific Academy of Higher Education and Research University, Udaipur-313003, Rajasthan, India Faculty of Pharmacy, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India The study evaluated the preliminary phytochemical properties, isolation, characterization, antioxidant, and antimicrobial activities of the methanol extract of Achyranthes aspera Linn. The plant material was collected, extracted, and subjected to thin-layer chromatography and preliminary phytochemical analysis. The antioxidant and antibacterial properties of AAME were assessed using in-vitro techniques, including the 2,2-diphenyl-1-picrylhydrazyl assay and the agar gel plate method. Bioactive compounds were separated and characterized using Fourier-transform infrared spectroscopy. The Rf values were obtained 0.74, 0.68, 0.19, 0.20, 0.24, 0.36, 0.44 and 0.64, these Rf values data reference sources  indicated that these bioactive compounds are present in extract such as ferulic acid, caffeic acid, oleanolic acid, ursolic acid, Flavonoid-VII, Phenolic acid-VI, Flavonoid-III, and Flavonoid-I. After the Rf values confirmation were isolated a compound through the TLC method that was found to be derivatives of quercetin (Flavonoid-III). Isolated compound was confirmation using FTIR interpretation results. Additionally, the presence of the results demonstrated that AAME exhibited significant antioxidant activity, comparable to standard ascorbic acid. Moreover, its antibacterial activity against infection-causing bacterial strains was found to be comparable to that of a standard drug. These findings suggested that AAME, in its entirety, possesses potent antioxidant properties and could serve as a valuable natural source of antibacterial and antioxidant agents for topical applications. https://www.biotech-asia.org/vol22no1/preliminary-phytochemical-isolation-characterization-antioxidant-and-antimicrobial-activity-assessments-of-achyranthes-aspera-l/ Achyranthes aspera; Antioxidant; antibacterial; flavonoid content; phenolic content