eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2024-12-20 21 4 1409 1414 10.13005/bbra/3313 53684 article Deepak Kumar Malik 1 Vivek Singh 1 Rajesh Agnihotri 1 Meenu Rathi 2 University Institute of Engineering and Technology, Kurukshetra University, Kurukshetra Department of Botany, GMN College Ambala Cantt, Ambala Cantt, Haryana This research paper aims to investigate the ability of plant growth promoting rhizobacteria, Bacillus tropicus to degrade chlorpyrifos in soil. Plant growth promoting rhizobacteria (PGPR) have the ability to degrade various xenobiotic compounds, including pesticides and enhance plant growth. The bacterial isolate DK5 identified as Bacillus tropicus, showed biofilm production, exopolysaccharide synthesis and surfactant analysis under abiotic stress. Chlorpyrifos degradation by DK5 was examined using liquid phase extraction followed by HPLC. In HPLC analysis, DK5 degraded 96.1% of chlorpyrifos within 30 days under laboratory conditions. DK5 can be used for remediation of chlorpyrifos form pesticide contaminated soil. The inoculation of DK5 in pesticide contaminated soil can be a promising bioremediation technique for chlorpyrifos removal. https://www.biotech-asia.org/vol21no4/bioremediation-of-chlorpyrifos-contaminated-soil-by-exopolysaccharide-surfactant-and-biofilm-synthesising-plant-growth-promoting-rhizobacteria/ Abiotic-stress; Biofilm; Chlorpyrifos; HPLC; Exopolysaccharide; PGPR; Remediation; Surfactant