eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2024-09-30 21 3 1103 1110 10.13005/bbra/3289 52267 article Manish Kumar Yadav 1 Komal Sharma 1 Ajay Kumar Shukla 2 Faculty of Pharmacy, Bhupal Nobles' University, Udaipur, Rajasthan, India Institute of Pharmacy, Dr Rammanohar Lohia Avadh University, Ayodhya, India Aim and objective: The study aimed to use in-vitro DPPH and the HaCaT skin cancer cell line method to examine hydroalcoholic leaf extract's antioxidant and anticancer properties from Nerium oleander. Methodology: The hydroalcoholic extract of Nerium oleander was prepared to assess antioxidants and anti-cancers using the in-vitro DPPH and MTT assay method against the HaCaT skin cancer cell line. Results: The antioxidant activity of Nerium oleander hydroalcoholic leaves extract was found to be IC50 =896.9µg/ml, equivalent to IC50= 10.93µg/ml of ascorbic acid, and IC50 = 91.49±0.181µg/ml effective against the HaCaT skin cancer cell line. Further research against carcinogenesis from the hydroalcoholic extract of Nerium oleander leaves, which demonstrated therapeutic potential against cancer cells, can yield significant results. Conclusion: The bioactive chemicals of Nerium oleander leaves may be beneficial for treating skin cancer. Limitations: The study's emphasis on in-vitro tests, which might not accurately capture the nuances of in-vivo settings, has limitations. To confirm the extract's potential for medicinal use, more research should examine its safety and efficacy in animal models and human trials. https://www.biotech-asia.org/vol21no3/antioxidant-and-anticancer-activity-assessment-of-n-oleander-on-the-hacat-skin-cancer-cell/ Hydroalcoholic extract; HaCaT skin cancer cell line; N. oleander; Skin cancer