eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2024-07-01 21 2 765 777 10.13005/bbra/3263 51411 article Kinjal Gohil 1 Sureshkumar Dhakhda 1 Vipul Patel 2 Ajay Rathod 3 Pradeep Kumar Singh 1 Rai School of Sciences, Rai University, Ahmedabad India. Department of Chemistry, School of Energy Technology, Pandit Deendayal Energy University, Gandhinagar, Gujarat India. Department of Chemistry, KSKV Kachchh University, Bhuj 370015, Gujarat India. A reliable and green technique for the synthesis of silver nanoparticles has been developed using plant extracts and characterized by UV-visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD) and Scanning electron microscopy (SEM). This research presented a simple and effective approach to synthesizing silver nanoparticles and tested their antibacterial activities. Plant extracts were prepared from Azadirachta indica, Mangifera indica, Eucalyptus and Fenugreek. In the UV-visible spectra peaks found around 350-450 nm indicated successful synthesis of silver nanoparticles. The size and shape of synthesized silver nanoparticles were studied by Scanning Electron Microscopic analysis, where the size of silver nanoparticles was found around 15 to 20 nanometers and a spherical shape was observed. X-ray diffraction spectra demonstrated characteristic Bragg peaks, and confirmed the cylindrical, face-centered composition of the silver nanoparticles. Good antibacterial activity was shown against Gram-negative bacteria and moderate activity was shown in antifungal study. https://www.biotech-asia.org/vol21no2/green-synthesis-of-silver-nanoparticles-using-various-plant-extracts-and-evaluation-of-their-antimicrobial-activities/ Antibacterial; Antifungal Activity; Characterization; Green Synthesis; Silver Nanoparticles