eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2023-12-31 20 4 1511 1517 10.13005/bbra/3196 48701 article Saloni Ekka 1 Jonali Owary 1 Department of Biotechnology, Bodoland University, Kokrajhar, India Flax seed oil is a vegetable oils with a rich source of fatty acids especially omega-3-fatty acid α-linolenic acid. Extraction of flaxseed oil employes many techniques including solvent extraction. The present work estimated the effect of a total of six solvents, both polar and non-polar on the physio-chemical characteristics of flax seed oil. Amongst the polar solvents, isopropanol showed better iodine value and DPPH free radical scavenging values. Overall, hexane showed the highest oil content of 45% (w/w). This study indicated that hexane a non-polar solvent showed the most efficient indicators in terms of iodine value (IV) (25.3 ± 0.41 iodine/ 100 g oil), peroxide value (PV) (21.2 ± 0.7 meq KOH/kg), saponification value (SV) (203.0 mg KOH/g oil) as well as DPPH free radical scavenging activity at 76.7 ± 2.32 % compared to the other polar solvents. https://www.biotech-asia.org/vol20no4/extraction-effect-of-polar-and-non-polar-solvents-on-the-physio-chemical-characteristics-of-flaxseed-oil/ Flaxseed; Oil; Polar Non-Polar; Physiochemical Parameters; Solvent Extraction