eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2023-12-31 20 4 1449 1459 10.13005/bbra/3190 48961 article Abinash, R. 1 Karthick, A 1 Jegan, G 2 Department of Biotechnology, Dr. M.G.R. Education and Research Institute University, Maduravoyal, Chennai, Tamil Nadu, India Department of Biotechnology, Apollo Arts and Science College, Poonamallee, Chennai, Tamil Nadu, India. Antibiotics are one of our most important weapons in fighting with bacterial infections and diabetics. Drugs were derived from natural sources that play an important role in the treatment and prevention of human diseases. Traditional fermented foods containing lactic acid bacteria such as milk, yogurt, curd, etc., have historically been consumed by human. Lactobacilli are well known friendly bacteria for their probiotic activities against pathogens. In this investigation cow and goat milk samples were selected and collected for the study. The gram stain was used for morphological identification of Lactobacillus sp. in the two samples. The two isolated Lactobacillus sp. from goat and cow milk samples were subjected to the extraction of secondary metabolites using the solvent ethyl acetate. The two extracts of Lactobacillus spp. those were isolated from cow and goat milk samples, showed antibacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. The zone of inhibition was assessed by mm. The good results of zones were observed at 1000 and 2000 µg in cow milk and 2000 µg in goat milk extraction. These metabolites were also showed good results in antidiabetic activity using the alpha amylase inhibition activity. The IC₅₀ value has showed moderate activity in the two crude extractions when compared to acarbose standard drug. Therefore, in this investigation the Lactobacillus sp. producing secondary metabolites was good source for the activity of antibacterial and antidiabetic activity. https://www.biotech-asia.org/vol20no4/antibacterial-and-anti-diabetic-activities-of-ethyl-acetate-fractions-of-lactobacillus-isolated-from-cow-and-goat-milk/ Antimicrobial; α-amylase inhibition; Milk; Lactobacillus spp; Secondary Metabolites