eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2023-09-30 20 3 945 952 10.13005/bbra/3145 47325 article Vardhana Janakiraman 1 Kathiravan Govindarajan 2 Thenmozhi 1 Department of Biotechnology, Vels Institute of Science Technology and Advanced Studies, Pallavaram, Chennai-600117. India Department of Botany, RKM Vivekananda College, University of Madras, Chennai- 600004.India Nanotechnology is concerned with the creation and stabilisation of nanoparticles. The biological method necessitates the creation of nanoparticles that are eaten by microorganisms capable of digesting nanoparticles in various forms. The fungus Pestaloptiopsis breviseta is used in this study to demonstrate the extracellular production of stable silver nanoparticles. The fungal culture was isolated from a stable Catharanthus roseus (L) G.don leaf sample, a common therapeutic plant. They were produced after the AgNO3 solution was employed to treat the cell filtrate and the fungal mat at room temperature and in the dark. (1 mM). The cell filtrate made silver nanoparticles that were between 171-378 nm in size, whereas the fungal biomass was between 140-280 nm in size. The cell lines MCF-7 and A549 were likewise treated with the silver nanoparticles made by the fungi. GraphPad Prism 5 software was used to track the percentage of living cells for 24 and 48 hours at different concentrations of the MCF-7 and A549 cell lines based on the IC₅₀ value. https://www.biotech-asia.org/vol20no3/silver-nanoparticles-and-its-invitrovj1-cytotoxic-behaviour-a-fungi-aided-synthesis/ AgNP’s; A549; Cytotoxicity; Endophytic Fungi; MCF-7; Pestaloptiopsis Breviseta