eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2023-09-30 20 3 1039 1045 10.13005/bbra/3154 46746 article Rekha Adimulam 1 P Sujathamma 1 Department of Biosciences and Sericulture Sri Padmavati Mahila Visvavidyalayam (Women’s University) Tirupati-517502, Andhra Pradesh. The study focused on the molecular phylogenetics for the identification of genus Chara species through 18S rRNA genes. Genus Chara sample is collected in the freshwater streams of Talakona region, Chittoor district, Andhra Pradesh and DNA was isolated. The 18S rRNA, gene sequence was used to carry out BLAST with the ‘nr’ database of NCBI GenBank database. First ten sequences were selected based on maximum identity score using multiple alignment software program Clustal W. Ribosomal Database Project was used for generating distance matrix and Molecular Evolutionary Genetics Analysis 6 Software was used for constructing the phylogenetic tree. Based on the maximum identifying score Chara species was identified as Chara foetida. https://www.biotech-asia.org/vol20no3/18s-rrna-approach-for-identification-of-chara-l-species/ Chara foeitda; Identification; Molecular phylogenetics; Phylogenetic tree