eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2023-03-30 20 1 341 350 10.13005/bbra/3093 44880 article Rakesh Singh Chauhan 1 Rahasya Mani Mishra 2 Centre for Biotechnology Studies, Awadhesh Pratap Singh University, Rewa, 486003, Madhya Pradesh, India. Department of Environmental Biology, Awadhesh Pratap Singh University, Rewa, 486003, Madhya Pradesh, India. The study's goal was to investigate and characterize alkaline protease-producing Fermentibacillus sp. RSCVS-HS3. The bacterium Fermentibacillus sp. RSCVS-HS3 was isolated from Vindhya region (Rewa division) of Madhya Pradesh of central India. It grew on casein-containing media at pH 12 and protease activity was found positive. . The enzyme was in a stable state in its crude form at 50℃ and pH 12, indicating thermal stability and the alkaline nature of the enzyme. It was rod-shaped, long, filamentous, gram-positive bacterium and was positive for casein hydrolysis. Based on NCBI BLAST and Phylogenetic Analysis of 16s rRNA, it was identified as Fermentibacillus sp. RSCVS-HS3. This is probably the first study to date, stating the alkaline protease synthesis from any Fermentibacillus species. It was closest to Fermentibacilolus polygoni IEB3 but in contrast to this, the identified bacterium showed casein hydrolysis. This is probably the first study to date, stating the alkaline protease synthesis from any Fermentibacillus species. https://www.biotech-asia.org/vol20no1/investigations-and-characterization-of-alkaline-protease-producing-fermentibacillus-sp-rscvs-hs3/ Alkaline protease; Bacteria; Characterization; Fermentibacillus; Identification; Soil