eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2022-12-20 19 4 1009 1018 10.13005/bbra/3050 42658 article Amit Kumar Sehgal 1 Anand Sagar 1 Department of Biosciences, Himachal Pradesh University, Summer Hill, Shimla (H.P.) 171005 India This paper describes for the first time in vitro mycorrhization between the two wild edible boletes (Boletus edulis and Suillus sibiricus) with Pinus gerardiana. The synthesis was carried out in a controlled growth chamber using peat, vermiculite, fungal medium and mycelial inoculum of each fungi in test tubes. The test tubes were regularly observed for mycorrhization. The seedlings of P. gerardiana were picked after five months of inoculation to examine symbiotic association between its root system with B. edulis and S. sibiricus. The B. edulis formed dark reddish brown whereas S. sibiricus synthesized light brown orange coloured mycorrhizae. The transverse sections of synthesized mycorrhizae showed a well developed fungal mantle and Hartig net for both (B. edulis and S. sibiricus) ectomycorrhizal fungi tested. The mycorrhization has significant effect on the overall growth of seedlings as compared to control. https://www.biotech-asia.org/vol19no4/in-vitro-mycorrhization-of-two-wild-edible-bolete-species-with-pinus-gerardiana-an-economically-high-altitude-conifer/ Boletus edulis; Ectomycorrhiza; in vitro; Suillus sibiricus;