eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2022-09-29 19 3 727 735 10.13005/bbra/3024 42043 article Devaraj Dharmadevi 1 Punamalai Ganesh 1 Kandasamy Sivasubramani 1 Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar, Chidambaram - 608002, Tamil Nadu, India Chemical surfactants are non-biodegradable and harmful, thus researchers are looking for better alternatives. The present study aimed to isolate bioemulsifier producing bacteria from oil-contaminated sediments. Nearly, 19 morphologically distinct bacteria were isolated and screened for bioemulsifier producing potential. Based on the screening, one efficient isolate PHCS 7 was selected and further subjected to molecular identification. After characterization, the isolate was identified as Acinetobacter beijerinckii PHCS 7 and further employed for growth kinetic profiling and optimization of physical factors for bioemulsifier production. During 48hrs incubation, A. beijerinckii PHCS 7 showed 64.6% emulsification activity with 8.69g/L of cell biomass. Similarly, during the optimization study pH, 8 and temperature of 35°C favored 67.9% and 69.7% emulsification activity, respectively. The current research establishes a foundation for future research on cost-effective large-scale production. https://www.biotech-asia.org/vol19no3/delving-of-a-promising-bioemulsifier-producing-bacterium-from-an-oil-contaminated-coastal-site-and-its-enhanced-production/ Bioemulsifier; Growth kinetics profile; Oil-contaminated coast; Marine Acinetobacter beijerinckii; Optimization