Aplectana duttaphryni Sou, Sow and Nandi, 2014 was first reported in Kulti, Asansol coal-mine region, West Bengal from a toad, Duttaphrynus melanostictus (Schneider, 1899) only using light microscope. Present article describes, A. duttaphryni in details collected from the rectum of an anuran frog, Fejervarya limnocharis (Gravenhorst, 1829) in Birbhum district, West Bengal. Aplectana duttaphryni is reported for the first time from Birbhum district, West Bengal. The parasites identified by its lanceolate shaped gubernaculum, presence of 1 pair of similar and equal spicules and also with the presence of 3 pairs of preanal papillae, 2 pairs of adanal papillae, 13 pairs of postanal papillae and single papillae on the upper lip of anus. In the present study, Birbhum district recorded as new geographical locality and F. limnocharis recorded as a new host.