eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2021-08-30 18 2 287 295 10.13005/bbra/2915 38978 article Turki M. Dawoud 1 Fatimah Alshehrei 2 Khaizran Siddiqui 3 Fuad Ameen 1 Jamila Akhtar 3 Afsheen Arif 4 Department of Botany and Microbiology, College of Science, King Saud University, Riyadh11451, Saudi Arabia Department of Biology, Jumum college university, Umm Al-Qura University, P.O Box 7388, Makkah 21955, Saudi Arabia Center of Molecular Genetics, University of Karachi, Pakistan. Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan Background: The wide use of dextran in many different applications, makes its industrial production a challenge and, hence, to obtain a control branched structure of this enzyme research is in progress. Objectives: In the present paper, the enzyme dextransucrase, produced by cultivation of the bacterium Leuconostoc mesenteroides CMG713, was purified and characterized. Methods: The produced dextransucrase was partially purified by PEG400 obtaining a purification factor of 29.4-fold and an overall yield of 18.3% from the initial crude enzymatic extract. Results: The partially purified dextransucrase had a specific activity of 24.0 U/mg and presented a molecular weight of about 200 kDa. In addition, the produced dextransucrase was stable at 30ºC and pH 5.5 for 3 days and led to a highly soluble dextran with wide potential industrial applications.  The current study has successfully partial purification, characterization and conformation of dextransucrase produced by fermentation of the bacterium Leuconostoc mesenteroides CMG713. https://www.biotech-asia.org/vol18no2/purification-characterization-and-n-terminal-protein-sequencing-of-the-enzyme-dextransucrase-produced-by-leuconostoc-mesenteroides/ Dextran; Dextransucrase; Leuconostoc m esenteroides