eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2021-08-30 18 2 395 401 10.13005/bbra/2926 39133 article Sahare KN 1 Department of Microbiology, Faculty of Life Sciences, Guru Nanak College Of Science, Ballarpur, (Gondwana University), Dist.- Chandrapur (Maharashtra State), India 442 701. In the present work antifilarial active fraction was isolated from the leaves Chloroform extract of Aegle marmelos Corr.  evaluated in vitro for antifilarial activity and studied the possible oxidative role against Setaria cervi parasite.   Antifilarial study was carried out with isolated fractions by worm motility and MTT assays. Complete parasite motility inhibition was observed at 0.002 to 0.08 mg/mL in motility assay and in MTT assay plant fraction gave > 50% reduction 58.9, 74.6 and 97.2% at concentrations 0.02, 0.04 and 0.08 mg/mL at 10, 6 and 2 hours incubation period respectively (p< 0.05). Inhibitory concentration (IC50) was found to be 0.015 mg/mL. Oxidative parameters levels for MDA, Carbonyl content and Nitric oxide were identified as antifilarial activity achieved. The level of oxidative parameters was calculated in dose dependent manners as compared to the control level.  The antifilarial activity of isolated fraction is associated with the oxidative mechanism in this study. https://www.biotech-asia.org/vol18no2/antifilarial-screening-and-oxidative-role-of-isolated-fraction-from-aegle-marmelos-corr-leaves-extract/ Antifilarial; Aegle marmelos; leaves; Oxidation