eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2020-09-25 17 3 467 477 10.13005/bbra/2850 36732 article Jaciara S. de Araújo 1 Juliene da C. Rocha 1 Marcos A. O. Filho 1 Vitor T. Ribeiro 1 C. de P. Vasconcelos 1 Nathália K. de Araujo 2 Biochemical Engineering Laboratory, Chemical Engineering Department, Centre of Technology, Federal University of Rio Grande do Norte, 59072-970 Natal, Brazil Pharmacy Deparment, Federal University of Rio Grande do Norte, Av. General Gustavo Cordeiro de Farias S/N, Petrópolis, 59012-570, Natal – RN, Brazil. Rhamnolipids are biosurfactants synthesized by different species of microorganisms. In this study, the influence of carbon/nitrogen ratio (C/N) and percentage of inoculum on rhamnolipid production by Pseudomonas aeruginosa AP029-GLVIIA using glucose as substrate was evaluated. The critical micellar concentration (CMC) and surface tension were analyzed for the highest biosurfactant concentration, which presented values of 49.63 mg/L and 29.5 mN/m, respectively. Emulsification rates were determined for different solvents and showed the bioproduct's ability to form stable emulsions for up to 90 days. The efficiency of the biosurfactant in removing petroleum present in the sand was 16.8% and the antimicrobial activity of the rhamnolipid against fungal species was determined, showing its potential to inhibit fungi of the species Candida tropicalis and Candida albicans. https://www.biotech-asia.org/vol17no3/production-of-rhamnolipids-by-pseudomonas-aeruginosa-ap029-glviia-and-application-on-bioremediation-and-as-a-fungicide/ Antifungal Activity; Bioremediation; Emulsification; Pseudomonas aeruginosa; Rhamnolipid