eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2020-09-25 17 3 587 599 10.13005/bbra/2862 36697 article Lekshmi K. Edison 1 Vipin Mohan Dan 1 Reji S. R. 1 Pradeep N. S. 2 Microbiology Division, KSCSTE-Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram, Kerala- 695 562, India KSCSTE-Malabar Botanical Garden and Institute of Plant Sciences, Kozhikode, Kerala- 673014, India β-glucan rich cereals such as barley and oats serve as a raw material in breweries and also used as an animal feed. Digestion of β-glucan is often a major hurdle, thus providing exogenous enzyme β-glucanase serves as an option. The present study takes an effort for the expression and over production of β-glucanase genes from Streptomyces sp in E. coli. The exo-β-1,4-glucanase and endo-β-1,3-glucanase encoding genes were isolated and codon optimized and significant-high expression levels were obtained in E. coli strain. The expressed enzymes showed broad pH stability, good thermostability, and better affinity towards the barley β-glucan substrate. The study implies that heterologous expression with codon optimization strategy enhances the production of Streptomyces origin beta-glucanase enzymes with prominent physio-chemical properties for efficient beta-glucan degradation. https://www.biotech-asia.org/vol17no3/a-strategic-production-improvement-of-streptomyces-beta-glucanase-enzymes-with-aid-of-codon-optimization-and-heterologous-expression/ Animal Feeds; Brewing; Beta Glucanase; Industrial Enzymes; Production Enhancement; Streptomyces; Western Ghats