eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2019-12-28 16 4 779 787 10.13005/bbra/2794 34805 article Anan Kalakattawi 1 Sana G. Al Attas 1 Sherif Edris 1,2,3* Ahmed Z. Abdel Azeiz 4 Ahmad F. AlGuthami 5 Ahmed G. Hegazi 6 Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia. Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia. Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia.Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt .Princess Al-Jawhara Al-Brahim Centre of Excellence in Research of Hereditary Disorders (PACER-HD), Faculty of Medicine, King Abdulaziz University (KAU), Jeddah, Saudi Arabia College of Biotechnology, Misr University for Science and technology (MUST), Egypt. Al Guthami Foundation, Jeddah, Saudi Arabia. National Research Centre, Giza, Egypt Candida albicans is a common human yeast that infect several epithelial tissues including vagina. The increase of  drug-resisting C. albicans encouraged the researchers to find alternative treatment. Honey medical signatures such as bactericidal, antifungal and anti-candida made it a possible candidate for disease treatment. In addition, rose essential oil possesses a wide range of  biochemical activities in folkloric medicine including anti-microbial activities. The present research utilizes honey alone or in conjunction with Taifi rose (Rosa damascena) oil as anti-candida agent to treat vaginal candidiasis. Three local monof oral honeys from different flower sources and/or geographic origins were tested with four concentrations (50, 80 and 95%), while two concentrations of  the Taifi rose oil (1 and 2%). anti-candida activity of  honey  alone or in conjunction with Taifi rose oil  was determined as well as phenolic and flavonoids contents were determined. Also, GC-MS analysis of  volatile oils and alkaloids were evaluated. The results of  this study indicated that acidity is within the allowed range for commercialization and long-lasting storage. All honeys tested inhibited completely the C. albicans growth at concentrations 80% and 95% either incubation after 48 or 72 h. Also, only Markh and Manuka honeys were completely inhibited C. albicans growth at 50% concentration. Also, C. albicans growth inhibited completely at 2% Taifi rose oil after the incubation periods of  48 and 72 h. The phenolic compounds and flavonoids were analysed by mass spectrometry analysis which revealed the Markh honey showed the presence of  gallic acid and quercetin that proved to have antifungal activity. It could be concluded that mixed Markh honey and Taifi rose oil treatment was capable to inhibit C. albicans growth completely. Further research is required to determine the anti-candida activity of  the mixture of  Markh honey and Taifi rose oil in the human body as a new therapeutic drug to treat vaginal candidiasis. https://www.biotech-asia.org/vol16no4/in-vitro-anti-candida-activity-of-different-saudi-honeys-and-honey-mixed-with-taifi-rose-oil/ Flavonoids;  Phenols; Vaginal Candidiasis