eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2018-12-25 15 4 851 860 10.13005/bbra/2694 32643 article Raman Jasrotia 1 Jyotdeep K. Raina 2 Minakshee Sharma 2 Rakesh K. Panjaliya 1 B.R. Kundal 3 Parvinder Kumar 1,2 Department of Zoology, University of Jammu, Jammu, 180006, India. Institute of Human Genetics, University of Jammu, Jammu, 180006, India. Department of Neurology, Govt. Super Speciality Hospital, Jammu, India. Disturbance in vascular functioning pathways has been related to pathophysiology of migraine. The present study investigated the role of MTHFR C677T and ACE I/D gene polymorphisms in migraine susceptibility within the population of Jammu province of J&K state. A sum of 252 subjects including 102 migraine patients and 150 non-migrainous unrelated healthy controls were enrolled for the present study. PCR-RFLP was performed for determining MTHFR gene variations. For detecting insertion/deletion in ACE gene PCR was performed. In case of MTHFR, ‘T’ allele (variant allele) and TT genotype (variant) was found to be present only in migraine patients but not in controls thereby suggesting its positive role in migraine pathophysiology. For ACE I/D polymorphism, higher frequency of DD genotype (32.35 % vs 15.3 %) and D allele (0.51 vs 0.4) were observed in patients than in controls. Logistic regression analysis revealed a significant association of ACE I/D polymorphism with risk of migraine. However, a direct link of MTHFR C677T polymorphism with migraine risk was not found. https://www.biotech-asia.org/vol15no4/relationship-of-mthfr-and-ace-gene-variations-with-migraine-susceptibility-a-case-control-study-in-the-population-of-north-india-jammu/ Angiotensin Converting Enzyme; Genotype; Migraine Methylenetetrahydrofolate Reductase; Polymorphism