eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2018-12-25 15 4 887 898 10.13005/bbra/2699 32316 article Antony V. Jenila 1 J. Joel Gnanadoss 1 Microbial and Environmental Biotechnology Research Unit, Department of Plant Biology and Biotechnology, Loyola College, Chennai 600 034, Tamil Nadu, India. Endophytic fungi Fusarium sp. LCJ273 capable of producing L-asparaginase was isolated from the medicinal plant Adhatoda vasica. The aim of the present study was to maximize L-asparaginase production by submerged fermentation through statistical optimization. L-Asparaginase production by Fusarium sp. LCJ273 was studied in five different media. Various nutritional parameters specifically carbon, nitrogen and inducers were optimized for enhancing the production of L-asparaginase. In addition, different statistical based experimental designs were also applied to increase the production of L-asparaginase by Fusarium sp. LCJ273. Dextrose, ammonium sulphate and wheat bran were found to be effective for growth and higher yield of L-asparaginase in Modified Czapek’s Dox Broth. Dextrose at a concentration of 3.0 g/L increased L-asparaginase production up to 9.18±0.9 U/mL, ammonium sulphate at the concentration of 20 g/L showed maximum L-asparaginase production up to 13.69±0.4 U/mL and wheat bran at 2.5 g/L yielded up to 14.24±0.5 U/mL. The maximum L-asparaginase production was observed by Fusarium sp. LCJ273 on 5^th day. The study revealed that through optimization, a 2 fold increase in L-asparaginase could be achieved. https://www.biotech-asia.org/vol15no4/formulation-of-a-suitable-medium-and-its-optimization-for-maximizing-l-asparaginase-production-from-endophytic-fungi-fusarium-sp-lcj273/ Endophytic Fungi; Enzyme Production; L-Asparaginase; Submerged Fermentation