eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2018-12-25 15 4 981 986 10.13005/bbra/2710 32369 article Mohamed.E.Osman 1 Om-kolthoum.H.Khattab 1 Abo Elnasr .A .A 1 Abdel Basset. S. 1 Department of Botany and Microbiology, Faculty of Science, Helwan University, Cairo, Egypt. A microbial fuel cell (MFC) has great potential for azo dyes decolorization and electricity generation by using filamentous fungi as biocatalysts. In this study, Aspergillus niger and Trichoderma harzianum were inoculated in anode chamber of double-chamber MFC to decolorize azo dye acid black 172 with Potassium Ferricyanide in the cathode chamber. During MFC operations, Acid black 172 oxidized and produced a maximum open-circuit voltage of 890 mV, and maximum current density of 163 mA/m² with an external resistance of 1000Ω. Also, variable parameters such as dye concentration, Co-substrate and dye as a sole carbon source were studied to improve microbial fuel cell performance. https://www.biotech-asia.org/vol15no4/acid-black-172-dye-decolorization-and-bioelectricity-generation-by-microbial-fuel-cell-with-filamentous-fungi-on-anode/ Acid Black 172; Bioelectricity; Decolorization; Microbial Fuel Cell