eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2018-09-25 15 3 687 698 10.13005/bbra/2677 31070 article S. Irudaya Monisha 1 J. Rosaline Vimala 1 Research Department of Chemistry, Holy Cross College, Affiliated to Bharathidasan University, Tiruchirapalli, Tamil Nadu - 620 002, India. Plants are made up of chemicals of differing nature produced by metabolism. These phytochemicals show varied biological activity. The present work was aimed to identify such active principle present in the stem bark of Manilkara hexandra (Roxb.)Dubard. Proximate, fluorescence, histochemical, mineral analysis was carried out for the plant material. From the preliminary analysis, the flavonoid rich extract was identified which was then subjected to using chromatographic methods for compound separation. Characterization of the separated compound was done by UV, FT-IR, and GC-MS studies. 7,9- DI-TERT-BUTYL-1-OXASPIRO[4.5]DECA-6,9-DIENE-2,8-DIONE was identified by GC-MS analysis. Antibacterial activity was carried and it shows high zone of inhibition, when concentration increases from 30 to 150 μg/ml. The separated flavanoid in future could be used to study its wide pharmacological applications as a lead compound in drug delivery. https://www.biotech-asia.org/vol15no3/extraction-identification-and-pharmacological-evaluation-of-phyto-active-compound-in-manilkara-hexandra-roxb-dubard-stem-bark/ Fluorescence; Histochemical; Manilkara Hexandra; Mineral Analysis Proximate