eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-12-25 14 4 1259 1268 10.13005/bbra/2568 28320 article Basma Al-Johani 2 Amna N. Khan 2 Zahra M. Alamshany 2 Munazza Gull 3 Elham S. Azam 2 Samia A. Kosa 2 Center of Excellence in Environmental Studies, King Abdulaziz University, Jeddah, Saudi Arabia. Department of Chemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia. Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia. The colloidal dispersion of copper nanoparticles (CuNPs), prepared by reducing Cu²⁺ ions using ascorbic acid, was characterized and used for electrochemical and antimicrobial activity investigations. By depositing CuNPs onto the glassy carbon electrode (GCE) surface the CuNPs/GCE was constructed, which was used to study electrochemical behavior of CuNPs and to carry out direct electrochemical detection of trichloroacetic acid (TCA) and 2-chlorophenol (2-CP) in neutral medium. Excellent electrocatalytic ability of CuNPs, assessed by cyclic voltammetry (CV), for the reduction of TCA and 2-CP was detected. The electrochemical impedance analysis (EIS) of the GCE and CuNPs modified GCE evidenced higher charge transfer activity across the modified electrode surface. The antibacterial activity tests of as-synthesized CuNPs on the selected pathogenic strains of pathogenic strains of Salmonella group B (7.9±0.912), Klebsiella pneumonia (8.33±1.561), Escherichia Coli (15.65±1.612), Enterococcus faecalis (5.4±0.612), Staphylococcus aureus (12.6±1.531) and yeast Candida albicans (11.4.3±1.512), respectively, were performed. The results indicated that the use of CuNPs can be pursued as an alternative strategy (to antibiotics) for averting infections by controlling bacterial adhesion and bacterial bio-film formation against microbial infections. https://www.biotech-asia.org/vol14no4/synthesis-electrochemical-and-antimicrobial-activity-of-colloidal-copper-nanoparticles/ Aqueous Dispersion; Antimicrobial Activity; CuNPs;  Electrochemical Detection; Enterococcus Faecalis