eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-12-25 14 4 1401 1407 10.13005/bbra/2585 28072 article Bantanahal Talari Vishwanatha 1 Girish Babu K 2 Padmashri 1 Swathi B Malagi 1 Chethan J Dandin 2 Sreenivasa Nayaka 1 Department of Botany, Karnatak University, Dharwad-580003, Karnataka, India. Department of Microbiology and Biotechnology, Karnatak University, Dharwad-580003, Karnataka, India. The present investigation is carried out for the isolation and molecular characterization of actinomycetes from the soil samples from Karnatak University campus, Dharwad, Karnataka, India. Totally four actinomycetes isolated, out of four isolated only one actinomycetes strain (SN-2) showed positive results for antimicrobial activity. SN-2 isolated actinomycetes was further analysed for morphological, physiological, biochemical and 16S ribosomal RNA gene sequencing. The SN-2 strain gene sequence was predicted with secondary structure and restriction sites were identified with the help of Genebee and NEBcutter tools. This investigation clearly indicates that the isolated strain SN-2 as Streptomyces sp. https://www.biotech-asia.org/vol14no4/isolation-identification-and-characterization-of-streptomyces-sp-sn-2/ Actinomycetes; Streptomyces; Antimicrobial; 16S rRNA; Cross streak method