eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-09-25 14 3 989 996 10.13005/bbra/2533 27046 article Sri Rahayu 1.2 Maggy T. Suhartono 2 Wardhana Suryapratama 1 Muhamad Bata 1 Faculty of Animal Science, Jenderal Soedirman University,Purwokerto-Indonesia. Faculty of Agricultural Technology, Bogor Agricultural University, Bogor-Indonesia. Edible bird’s nest (EBN) as a kind of functional food has high economic value depending on the quality such as color and hygiene. The purpose of this research was to find optimum condition for application of keratinolytic enzymes Bacillus sp. MTS in cleaning EBN. Activating agents for both enzymes were cation divalents, EDTA, reducing agents, organic solutions, and antibacterial agents. Additives compound that able to increase keratinase activity were used to make cleaning solution and its tested on EBN and human hair. Alcoholic solutions (25% ethanol, 25% methanol, 25% glycerol), and some divalent metallic ions(Ca²⁺, Mg²⁺, Mn²⁺, Zn²⁺)were able to increase keratinase while disulfide reductase was solely activated by 0.05 mM EDTA. The activity of both enzymes was inhibited by Na Cland Na-azide. The activity of keratinase of Bacillus sp. MTS in cleaning solution formulated in this research was 2-3 fold as much as control (crude extract) in human hair substrates. Gliserol and cations divalent increasing 2-3 fold keratinase activity in cleaning solution. The solution was successfully apllied to cleaning EBN with weight loss 2.3-2.5% approximately. https://www.biotech-asia.org/vol14no3/keratinolytic-enzymes-for-cleaning-edible-birds-nest/ Bacillus; cleaning; edible bird’s nest keratinase;