eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-06-28 14 2 615 620 10.13005/bbra/2485 25436 article Hossein Nazarian 1 Amir Hossein Ghadimi 2 Department of Agricultural Biotechnology, Payame Noor University, Karaj, Iran. Department of Food Science and Technology, Yasooj Branch, Islamic Azad University, Iran. In this study bulbetets of royal lily have been used as explants. The innovative approach is based on optimal placement and adventitious sprout induction and the adventitious sprout proliferation in special conditions. Then effects of different parts of scales and placement method, types and concentrations of cytokinin, different parts of bulbetet scales and placement method on adventitious sprout induction have been discussed. At the end the effects of plant growth regulators on induction and multiplication of bulbetet scales of royal lily have been studied. The results represents that the regeneration ability of outer layer scales of royal lily is better than the middle ones, which was better than the inner ones. The adaxial side of royal lily bulbetets in contact with medium upward can produce more and stronger adventitious sprouts than the adaxial side downward. https://www.biotech-asia.org/vol14no2/innovative-optimal-approach-to-implement-fast-propagation-tissue-culture/ adventitious sprout fast propagation;  Optimal placement; tissue culture;