eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-06-28 14 2 587 591 10.13005/bbra/2482 25824 article K. Mchiouer 1 S. Bennani 1 N. Sh. El-Gendy 2 M. Meziane 1 Department of Biology, Faculty of Sciences, Laboratory of Water Sciences, Environment and Ecology, University Mohammed I, Oujda, Morocco. Petroleum Biotechnology Laboratory, Department of Process Development, Egyptian Petroleum Research Institute, Nasr City, Cairo, Egypt. The aim of this study is to determine the microbiological quality of raw cows’ milk of Oujda city. Raw milk samples are collected randomly between June 2014 and May 2015 from 20 Mahlaba (dairies) for microbiological evaluation. The samples are analyzed to determine total mesophilic aerobic bacteria (TMAB), total coliform, fecal coliform, staphylococcus aureus, fecal streptococci, proteolytic bacteria and lactic acid bacteria. The results of bacterial count showed that there is a variation between all the milk samples and a period effect is also observed. The mean counts of total mesophilic aerobic bacteria from all sale points are between 1.76×10⁶ and 40.17×10⁶ CFU/ml. Milk samples reveled counts total coliform and fecal coliform ranging from 0.58×10⁵ to 11.10×10⁵ CFU/ml and from 0.60×10³ to 14.64×10³ CFU/ml, respectively. Staphylococcus aureus are also detected in all samples with counts ranging from 0.35 ×10³ to 3.08×10³ CFU/ml. Fecal streptococci are found in all milk samples, at counts ranging from 0.16 ×10² to 2.18 ×10² CFU/ml. And finally, proteolytic and lactic acid bacteria are between 0.3×10³ and 2.86×10³ CFU/ml and 2.37×10⁶ and 24.14 ×10⁶ CFU/ml respectively. These results indicate a lack of compliance with good manufacturing practice at milking, collection and transportation of raw milk. https://www.biotech-asia.org/vol14no2/evaluation-of-the-hygienic-quality-of-raw-cows-milk-in-oujda-city-morocco/ Raw milk; Hygiene; Microbiological quality; Pathogens; Oujda; Morocco