eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-03-25 14 1 13 23 10.13005/bbra/2412 22719 article Farah J. Hashim 1 Suaad M. Hussain 2 Muayad S. Shawkat 1 Department of Biotechnology /College of Science/ University of Baghdad/ Baghdad/ Iraq. Department of Chemistry /College of Science/ University of Baghdad/ Baghdad/ Iraq. Coumarin is a phenolic natural product extracted from yellow clover Melilotus officinalis by maceration in ethanol solvent. Crude extract was analyzed by high performance liquid chromatography then divided into two portions, first one  characterized by Fourier transform infrared spectroscopy (FTIR) and investigated for anticoagulation activity by prothrombin time (PT) while the second portion subjected for fractionation using column chromatography (CG) and fractions were characterized by FTIR, each fraction was detected as anticoagulant agent. As results, coumarin crude extract eluted with retention time (7.6 min.) as standard coumarin by HPLC, molecular spectrums of fractions obtained by column chromatography were measured using FTIR. Anticoagulation activity of crude extract results were dose dependent pattern .We concluded that M. officinalis is a good source for coumarin and coumarin derivatives as shown in (HPLC) analysis while using column chromatography (CG) and (FTIR) considered as valuable techniques for separation and characterization . https://www.biotech-asia.org/vol14no1/separation-characterization-and-anticoagulant-activity-of-coumarin-and-its-derivatives-extracted-from-melilotus-officinalis/ Anticoagulant; column chromatography coumarin;   FTIR; HPLC; Melilotus officinalis;