eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-03-25 14 1 373 380 10.13005/bbra/2455 21594 article Omchand Singh 1 Parmjit S. Panesar 1 Harish K. Chopra 2 Biotechnology Research Laboratory, Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal-148106, Punjab, India. Department of Chemistry, Sant Longowal Institute of Engineering and Technology, Longowal-148106, Punjab, India. In this present investigation, eleven bacterial cellulose producing isolates were selected out of 34 isolates from different sources. Among the eleven isolates the C18 isolate has been identified as a potential bacterial cellulose producer. Physiological and biochemical tests were carried out to identify the bacteria. The molecular characterization of C18 strain was done by 16 S rDNA analysis and identified as Gluconacetobacter xylinus due to 94% sequence similarity. The maximum bacterial cellulose production (3.96g/L) was obtained after incubation time of 168 h with Hestrin and Hchramm (HS) media in static culture.  Structural elucidation of bacterial cellulose was achieved using analytical techniques like FTIR, SEM and X-ray diffraction analysis. https://www.biotech-asia.org/vol14no1/isolation-and-characterization-of-cellulose-producing-bacterial-isolate-from-rotten-grapes/ Gluconacetobacter xylinus; bacterial cellulose; FTIR; SEM; X-ray diffraction