eng Oriental Scientific Publishing Company Biosciences Biotechnology Research Asia 0973-1245 2017-03-25 14 1 123 128 10.13005/bbra/2426 21305 article N. Abubacker 1 T. Deepalakshmi 1 Department of Biotechnology, National College, Tiruchirappalli 620 001, Tamil Nadu, India. Annona muricata L. (Annonaceae) commonly called ‘soursop’ also called prickly custard apple has been used in natural medicine in treating respiratory and digestive ailments. The plant has a poor fruit development and seed setting properties. To bring about proper conservation of this tree, in vitro plantlet regeneration through direct shoot regeneration and rooting protocol was achieved. In vitro callus production was achieved in leaf base explants on MS + BAP 1.5 mg/L+ 2,4-D 2.0 mg/L, callus cell morphogenesis indicated the establishment of proembryo, perfect dichotomy and shoot apices. The axillary shoot formation from nodal explants was achieved on MS + BAP 1.0 mg/L+ Kin 0.5 mg/L+ IBA 1.5 mg/Land rooting in MS + IBA 2.0 mg/L+ NAA 0.5 mg/L+ BAP 1.0 mg/L. The plantlets were acclimatized and thrived in greenhouse and then in natural environmental conditions. https://www.biotech-asia.org/vol14no1/in-vitro-direct-regeneration-of-annona-muricata-l-from-nodal-explant/ Annona muricata L; in vitro culture; morphogenesis